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Price discounts are the largest expenditure for smokeless tobacco as well, with tobacco companies spending 296.6 million on price discounts paid to retailers (an increase of 4% from 2019) and $85.3 million on price discount paid to retailers, representing a combined 67.4% of all marketing expenditures for smokeless tobacco

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Samples were stained for AIF and EndoG with the same primary antibodies as for western blotting, followed by Alexa Fluor 488 (A-21206, ThermoFisher Scientific, Carlsbad, CA) labeled anti-rabbit antibodies

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